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R&D Systems goat anti-clec11a antibody
Goat Anti Clec11a Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti-clec11a+antibody/goat+anti+clec11a+antibody/us11890320-777-20-23
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goat anti-clec11a antibody - by Bioz Stars, 2026-10

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Article Title: CLEC11A is a bone growth agent
Article Snippet: Sections were blocked in PBS with 10% horse serum for 30 minutes and then stained overnight at 4° C. with goat anti-Clec11a antibody (R&D systems, 1:500), rabbit anti-Aggrecan antibody (Chemicon, 1:500), rabbit anti-Perilipin antibody (Sigma, 1:2000) or goat anti-Osteopontin antibody (R&D, 1:500).

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( A, B ) The human ( A ) and mouse ( B ) Osteolectin proteins contain RGD and LDT sequences. ( C ) Alignment of Osteolectin amino acid sequences shows that the RGD and LDT domains are evolutionarily conserved among bony vertebrates. ( D, E ) RNA-seq analysis of integrin α ( D ) and β ( E ) subunits in PDGFRα + CD45 - Ter119 - CD31 - bone marrow stromal cells from enzymatically dissociated adult bone marrow (n = 2 independent samples). These cells are uniformly positive for LepR expression . ( F ) RNA-seq analysis of Itga1 , Itga6 , Itga11 , and Itgav in PDGFRα + CD45 - Ter119 - CD31 - bone marrow stromal cells, VE-Cadherin + bone marrow endothelial cells, and whole bone marrow cells (n = 2 independent samples per cell population). ( G ) Itga11 expression in cell populations from mouse bone marrow by qRT-PCR (n = 3 independent samples per cell population). The markers used for the isolation of each cell population are shown in . ( H ) In MC3T3-E1 preosteoblast cells expressing Flag-tagged Osteolectin, anti-Flag antibody co-immunoprecipitated endogenous integrin β1 and integrin α11 with Flag-tagged Osteolectin (results are representative of two independent experiments). ( I ) Recombinant human Osteolectin (rhOln) selectively bound to recombinant human integrin α 11 β 1 and α 10 β 1 , but not to other integrins (n = 3 independent experiments). ( J ) Integrin α11β1 bound Osteolectin and recombinant human Pro-Collagen 1α (rhCol1A) with similar affinities, but not bovine serum albumin (BSA) (n = 3 independent experiments). ( K ) Osteolectin, but not Pro-Collagen 1α, promoted osteogenic differentiation by MC3T3-E1 cells and human bone marrow stromal cells (n = 3 independent experiments). ( L ) 200 nM RGDS peptide inhibited the binding of integrin α11β1 to recombinant human Osteolectin. ( M ) 100 μM RGDS peptide inhibited osteogenic differentiation by MC3T3-E1 cells and human bone marrow stromal cells in response to 30 ng/ml of recombinant human Osteolectin. All numerical data reflect mean ±standard deviation. Statistical significance was determined with one-way ( G ) or two-way ANOVAs with Dunnett’s multiple comparisons tests ( K ) or Tukey’s multiple comparisons tests ( M ). 10.7554/eLife.42274.004 Figure 1—source data 1. Data for .

Journal: eLife

Article Title: Integrin alpha11 is an Osteolectin receptor and is required for the maintenance of adult skeletal bone mass

doi: 10.7554/eLife.42274

Figure Lengend Snippet: ( A, B ) The human ( A ) and mouse ( B ) Osteolectin proteins contain RGD and LDT sequences. ( C ) Alignment of Osteolectin amino acid sequences shows that the RGD and LDT domains are evolutionarily conserved among bony vertebrates. ( D, E ) RNA-seq analysis of integrin α ( D ) and β ( E ) subunits in PDGFRα + CD45 - Ter119 - CD31 - bone marrow stromal cells from enzymatically dissociated adult bone marrow (n = 2 independent samples). These cells are uniformly positive for LepR expression . ( F ) RNA-seq analysis of Itga1 , Itga6 , Itga11 , and Itgav in PDGFRα + CD45 - Ter119 - CD31 - bone marrow stromal cells, VE-Cadherin + bone marrow endothelial cells, and whole bone marrow cells (n = 2 independent samples per cell population). ( G ) Itga11 expression in cell populations from mouse bone marrow by qRT-PCR (n = 3 independent samples per cell population). The markers used for the isolation of each cell population are shown in . ( H ) In MC3T3-E1 preosteoblast cells expressing Flag-tagged Osteolectin, anti-Flag antibody co-immunoprecipitated endogenous integrin β1 and integrin α11 with Flag-tagged Osteolectin (results are representative of two independent experiments). ( I ) Recombinant human Osteolectin (rhOln) selectively bound to recombinant human integrin α 11 β 1 and α 10 β 1 , but not to other integrins (n = 3 independent experiments). ( J ) Integrin α11β1 bound Osteolectin and recombinant human Pro-Collagen 1α (rhCol1A) with similar affinities, but not bovine serum albumin (BSA) (n = 3 independent experiments). ( K ) Osteolectin, but not Pro-Collagen 1α, promoted osteogenic differentiation by MC3T3-E1 cells and human bone marrow stromal cells (n = 3 independent experiments). ( L ) 200 nM RGDS peptide inhibited the binding of integrin α11β1 to recombinant human Osteolectin. ( M ) 100 μM RGDS peptide inhibited osteogenic differentiation by MC3T3-E1 cells and human bone marrow stromal cells in response to 30 ng/ml of recombinant human Osteolectin. All numerical data reflect mean ±standard deviation. Statistical significance was determined with one-way ( G ) or two-way ANOVAs with Dunnett’s multiple comparisons tests ( K ) or Tukey’s multiple comparisons tests ( M ). 10.7554/eLife.42274.004 Figure 1—source data 1. Data for .

Article Snippet: Antibody , goat polyclonal anti-mouse Osteolectin , R and D Systems , AF3729 , (1:1000).

Techniques: RNA Sequencing, Expressing, Quantitative RT-PCR, Isolation, Immunoprecipitation, Recombinant, Binding Assay, Standard Deviation

( A ) Western blot of cell culture supernatant from parental or Osteolectin deficient MC3T3-E1 cells and human bone marrow stromal cells (hBMSC#1 and hBMSC#2 cells) (this blot is representative of blots from three independent experiments). ( B ) Osteogenic differentiation in culture of parental or Osteolectin deficient MC3T3-E1 cells, hBMSC#1 cells, and hBMSC#2 cells. Alizarin red staining was performed after 14 days (for MC3T3-E1 cells) or 21 days (for hBMSC cells) to quantify osteoblast differentiation and mineralization (n = 3 independent experiments). ( C ) qRT-PCR analysis of Dmp1 transcript levels in MC3T3-E1 cells, hBMSC#1 cells, and hBMSC#2 cells after 14 to 21 days of osteogenic differentiation (n = 5 independent experiments). ( D ) MC3T3-E1 cells, hBMSC#1 cells, and hBMSC#2 cells were stimulated with recombinant mouse Osteolectin (rOln) in osteogenic differentiation medium then lysed 0, 15, 30, or 60 min later and immunoblotted for phospho-PI3K, phospho-Akt, phospho-GSK3, β-catenin, total GSK3, and Actin (results are representative of 3 independent experiments). ( E ) MC3T3-E1 cells, hBMSC#1 cells, and hBMSC#2 cells were transferred into osteogenic differentiation medium for 24 hr with PBS or recombinant mouse Osteolectin, lysed, and immunoblotted for phospho-GSK3, β-catenin, and Actin (this blot is representative of blots from three independent experiments). ( F ) qRT-PCR analysis of Wnt target gene transcript levels ( Alkaline phosphatase , Axin2 , Lef1 , or Runx2 ) in MC3T3-E1 cells, hBMSC#1 cells, and hBMSC#2 cells 24 hr after transfer into osteogenic differentiation medium, with PBS or recombinant mouse Osteolectin (n = 5 independent experiments). ( G ) Parental or Osteolectin deficient MC3T3-E1 cells, hBMSC#1 cells, and hBMSC#2 cells after 24 hr in osteogenic differentiation medium were lysed and immunoblotted for phospho-GSK3, β-catenin, and Actin (this blot is representative of blots from three independent experiments). ( H ) qRT-PCR analysis of Wnt target gene transcript levels in parental or Osteolectin deficient MC3T3-E1 cells, hBMSC#1 cells, and hBMSC#2 cells 24 hr after transfer into osteogenic differentiation medium (n = 5 independent experiments). All numerical data reflect mean ±standard deviation. The statistical significance of differences was determined with two-way ANOVAs with Sidak’s multiple comparisons tests. 10.7554/eLife.42274.006 Figure 2—source data 1. Data for .

Journal: eLife

Article Title: Integrin alpha11 is an Osteolectin receptor and is required for the maintenance of adult skeletal bone mass

doi: 10.7554/eLife.42274

Figure Lengend Snippet: ( A ) Western blot of cell culture supernatant from parental or Osteolectin deficient MC3T3-E1 cells and human bone marrow stromal cells (hBMSC#1 and hBMSC#2 cells) (this blot is representative of blots from three independent experiments). ( B ) Osteogenic differentiation in culture of parental or Osteolectin deficient MC3T3-E1 cells, hBMSC#1 cells, and hBMSC#2 cells. Alizarin red staining was performed after 14 days (for MC3T3-E1 cells) or 21 days (for hBMSC cells) to quantify osteoblast differentiation and mineralization (n = 3 independent experiments). ( C ) qRT-PCR analysis of Dmp1 transcript levels in MC3T3-E1 cells, hBMSC#1 cells, and hBMSC#2 cells after 14 to 21 days of osteogenic differentiation (n = 5 independent experiments). ( D ) MC3T3-E1 cells, hBMSC#1 cells, and hBMSC#2 cells were stimulated with recombinant mouse Osteolectin (rOln) in osteogenic differentiation medium then lysed 0, 15, 30, or 60 min later and immunoblotted for phospho-PI3K, phospho-Akt, phospho-GSK3, β-catenin, total GSK3, and Actin (results are representative of 3 independent experiments). ( E ) MC3T3-E1 cells, hBMSC#1 cells, and hBMSC#2 cells were transferred into osteogenic differentiation medium for 24 hr with PBS or recombinant mouse Osteolectin, lysed, and immunoblotted for phospho-GSK3, β-catenin, and Actin (this blot is representative of blots from three independent experiments). ( F ) qRT-PCR analysis of Wnt target gene transcript levels ( Alkaline phosphatase , Axin2 , Lef1 , or Runx2 ) in MC3T3-E1 cells, hBMSC#1 cells, and hBMSC#2 cells 24 hr after transfer into osteogenic differentiation medium, with PBS or recombinant mouse Osteolectin (n = 5 independent experiments). ( G ) Parental or Osteolectin deficient MC3T3-E1 cells, hBMSC#1 cells, and hBMSC#2 cells after 24 hr in osteogenic differentiation medium were lysed and immunoblotted for phospho-GSK3, β-catenin, and Actin (this blot is representative of blots from three independent experiments). ( H ) qRT-PCR analysis of Wnt target gene transcript levels in parental or Osteolectin deficient MC3T3-E1 cells, hBMSC#1 cells, and hBMSC#2 cells 24 hr after transfer into osteogenic differentiation medium (n = 5 independent experiments). All numerical data reflect mean ±standard deviation. The statistical significance of differences was determined with two-way ANOVAs with Sidak’s multiple comparisons tests. 10.7554/eLife.42274.006 Figure 2—source data 1. Data for .

Article Snippet: Antibody , goat polyclonal anti-mouse Osteolectin , R and D Systems , AF3729 , (1:1000).

Techniques: Western Blot, Cell Culture, Staining, Quantitative RT-PCR, Recombinant, Standard Deviation

( A and B ) MC3T3-E1 cells, hBMSC#1 cells, and hBMSC#2 cells were transferred into osteogenic differentiation medium with PBS or 30 ng/ml recombinant mouse Osteolectin, as well as DMSO or 200 nM of the GSK3 inhibitor AZD2858. ( A ) Cells were lysed 24 hr later and immunoblotted for phospho-GSK3, β-catenin, and Actin. ( B ) Alizarin red staining after 14 days (MC3T3-E1 cells) or 21 days (hBMSC cells) to quantify osteoblast differentiation and mineralization (n = 3 independent experiments). ( C and D ) MC3T3-E1 cells, hBMSC#1 cells, and hBMSC#2 cells were transferred into osteogenic differentiation medium with PBS or 30 ng/ml Osteolectin, as well as DMSO or 200 nM of the β-catenin inhibitor IWR-1-endo. ( C ) Cells were lysed 24 hr later and immunoblotted for phospho-GSK3, β-catenin, and Actin. ( D ) Alizarin red staining after 14 days (MC3T3-E1 cells) or 21 days (hBMSC cells) to quantify osteoblast differentiation and mineralization (n = 3 independent experiments). All numerical data reflect mean ±standard deviation. The statistical significance of differences was determined with two-way ANOVAs with Tukey’s multiple comparisons tests. 10.7554/eLife.42274.008 Figure 3—source data 1. Data for Data for .

Journal: eLife

Article Title: Integrin alpha11 is an Osteolectin receptor and is required for the maintenance of adult skeletal bone mass

doi: 10.7554/eLife.42274

Figure Lengend Snippet: ( A and B ) MC3T3-E1 cells, hBMSC#1 cells, and hBMSC#2 cells were transferred into osteogenic differentiation medium with PBS or 30 ng/ml recombinant mouse Osteolectin, as well as DMSO or 200 nM of the GSK3 inhibitor AZD2858. ( A ) Cells were lysed 24 hr later and immunoblotted for phospho-GSK3, β-catenin, and Actin. ( B ) Alizarin red staining after 14 days (MC3T3-E1 cells) or 21 days (hBMSC cells) to quantify osteoblast differentiation and mineralization (n = 3 independent experiments). ( C and D ) MC3T3-E1 cells, hBMSC#1 cells, and hBMSC#2 cells were transferred into osteogenic differentiation medium with PBS or 30 ng/ml Osteolectin, as well as DMSO or 200 nM of the β-catenin inhibitor IWR-1-endo. ( C ) Cells were lysed 24 hr later and immunoblotted for phospho-GSK3, β-catenin, and Actin. ( D ) Alizarin red staining after 14 days (MC3T3-E1 cells) or 21 days (hBMSC cells) to quantify osteoblast differentiation and mineralization (n = 3 independent experiments). All numerical data reflect mean ±standard deviation. The statistical significance of differences was determined with two-way ANOVAs with Tukey’s multiple comparisons tests. 10.7554/eLife.42274.008 Figure 3—source data 1. Data for Data for .

Article Snippet: Antibody , goat polyclonal anti-mouse Osteolectin , R and D Systems , AF3729 , (1:1000).

Techniques: Recombinant, Staining, Standard Deviation

$( A ) Osteogenic differentiation in culture of parental or Itga11 deficient MC3T3-E1 cells, hBMSC#1 cells, and hBMSC#2 cells with PBS or recombinant mouse Osteolectin (n = 3 independent experiments). ( B ) qRT-PCR analysis of Dmp1 transcript levels in MC3T3-E1 cells, hBMSC#1 cells, and hBMSC#2 cells after 14 to 21 days of osteogenic differentiation (n = 5 independent experiments). ( C ) Parental or Itga11 deficient MC3T3-E1 cells, hBMSC#1 cells, and hBMSC#2 cells were transferred into osteogenic differentiation medium with or without recombinant mouse Osteolectin then lysed 24 hr later and immunoblotted for phospho-GSK3, β-catenin, and Actin (this blot is representative of blots from three independent experiments). ( D ) qRT-PCR analysis of Wnt target gene transcript levels in parental or Itga11 deficient MC3T3-E1 cells, hBMSC#1 cells, and hBMSC#2 cells 24 hr after transfer into osteogenic differentiation medium with PBS or Osteolectin (n = 5 independent experiments). ( E ) MC3T3-E1 cells, hBMSC#1 cells, and hBMSC#2 cells were transferred into osteogenic differentiation medium with PBS or 30 ng/ml Osteolectin, or 30 ng/ml recombinant Pro-Collagen 1α, then lysed 24 hr later and immunoblotted for phospho-GSK3, β-catenin, and Actin (this blot is representative of blots from three independent experiments). ( F ) qRT-PCR analysis of Wnt target gene transcript levels in MC3T3-E1 cells, hBMSC#1 cells, and hBMSC#2 cells 24 hr after transfer into osteogenic differentiation medium with PBS or 30 ng/ml Osteolectin, or 30 ng/ml Pro-Collagen 1α (n = 5 independent experiments). ( G ) Primary mouse bone marrow stromal cells were adherently cultured in osteogenic differentiation medium. PBS (control), recombinant human Pro-Collagen 1α, or recombinant human Osteolectin was added, then the cells were lysed 1 hr later and lysates were immunoblotted for phospho-FAK, phospho-GSK3, total FAK, and Actin (this blot is representative of blots from three independent experiments). ( H ) Primary mouse bone marrow stromal cells adherently cultured in osteogenic differentiation medium were treated with PBS (control), recombinant human Pro-Collagen 1α, or recombinant human Osteolectin then lysed 24 hr later and lysates were immunoblotted for phospho-FAK, phospho-GSK3, β-catenin, total FAK, and Actin (this blot is representative of blots from three independent experiments). ( I ) Primary mouse bone marrow stromal cells adherently cultured in osteogenic differentiation medium were treated with 30 ng/ml recombinant human Osteolectin or PBS (control) then lysed 2, 4, 6, 12, or 24 hr later. Nuclear and cytosolic/membrane-associated fractions were isolated from lysates by centrifugation then immunoblotted for β-catenin. As loading controls, Histone H3 was blotted in the nuclear fraction and Actin was blotted in the cytosolic/membrane-associated fraction (this blot is representative of blots from three independent experiments). ( J ) qRT-PCR analysis of Ctnnb1 transcript levels in cells from the experiment in panel ( I ) (n = 3 independent experiments). ( K ) Primary mouse bone marrow stromal cells from Lepr-Cre; Itga11 fl/fl or littermate control mice were adherently cultured in osteogenic differentiation medium. PBS (control), recombinant human Pro-Collagen 1α, or recombinant human Osteolectin was added then the cells were lysed 1 hr later and lysates were immunoblotted for phospho-FAK and FAK (this blot is representative of blots from two independent experiments). All numerical data reflect mean ±standard deviation. Statistical significance was determined with two-way ANOVAs with Tukey’s multiple comparisons tests ( A, B and D ), Dunnett’s multiple comparisons tests ( F ), or Sidak’s multiple comparisons tests ( J ). 10.7554/eLife.42274.010 Figure 4—source data 1. Data for .$

Journal: eLife

Article Title: Integrin alpha11 is an Osteolectin receptor and is required for the maintenance of adult skeletal bone mass

doi: 10.7554/eLife.42274

Figure Lengend Snippet: ( A ) Osteogenic differentiation in culture of parental or Itga11 deficient MC3T3-E1 cells, hBMSC#1 cells, and hBMSC#2 cells with PBS or recombinant mouse Osteolectin (n = 3 independent experiments). ( B ) qRT-PCR analysis of Dmp1 transcript levels in MC3T3-E1 cells, hBMSC#1 cells, and hBMSC#2 cells after 14 to 21 days of osteogenic differentiation (n = 5 independent experiments). ( C ) Parental or Itga11 deficient MC3T3-E1 cells, hBMSC#1 cells, and hBMSC#2 cells were transferred into osteogenic differentiation medium with or without recombinant mouse Osteolectin then lysed 24 hr later and immunoblotted for phospho-GSK3, β-catenin, and Actin (this blot is representative of blots from three independent experiments). ( D ) qRT-PCR analysis of Wnt target gene transcript levels in parental or Itga11 deficient MC3T3-E1 cells, hBMSC#1 cells, and hBMSC#2 cells 24 hr after transfer into osteogenic differentiation medium with PBS or Osteolectin (n = 5 independent experiments). ( E ) MC3T3-E1 cells, hBMSC#1 cells, and hBMSC#2 cells were transferred into osteogenic differentiation medium with PBS or 30 ng/ml Osteolectin, or 30 ng/ml recombinant Pro-Collagen 1α, then lysed 24 hr later and immunoblotted for phospho-GSK3, β-catenin, and Actin (this blot is representative of blots from three independent experiments). ( F ) qRT-PCR analysis of Wnt target gene transcript levels in MC3T3-E1 cells, hBMSC#1 cells, and hBMSC#2 cells 24 hr after transfer into osteogenic differentiation medium with PBS or 30 ng/ml Osteolectin, or 30 ng/ml Pro-Collagen 1α (n = 5 independent experiments). ( G ) Primary mouse bone marrow stromal cells were adherently cultured in osteogenic differentiation medium. PBS (control), recombinant human Pro-Collagen 1α, or recombinant human Osteolectin was added, then the cells were lysed 1 hr later and lysates were immunoblotted for phospho-FAK, phospho-GSK3, total FAK, and Actin (this blot is representative of blots from three independent experiments). ( H ) Primary mouse bone marrow stromal cells adherently cultured in osteogenic differentiation medium were treated with PBS (control), recombinant human Pro-Collagen 1α, or recombinant human Osteolectin then lysed 24 hr later and lysates were immunoblotted for phospho-FAK, phospho-GSK3, β-catenin, total FAK, and Actin (this blot is representative of blots from three independent experiments). ( I ) Primary mouse bone marrow stromal cells adherently cultured in osteogenic differentiation medium were treated with 30 ng/ml recombinant human Osteolectin or PBS (control) then lysed 2, 4, 6, 12, or 24 hr later. Nuclear and cytosolic/membrane-associated fractions were isolated from lysates by centrifugation then immunoblotted for β-catenin. As loading controls, Histone H3 was blotted in the nuclear fraction and Actin was blotted in the cytosolic/membrane-associated fraction (this blot is representative of blots from three independent experiments). ( J ) qRT-PCR analysis of Ctnnb1 transcript levels in cells from the experiment in panel ( I ) (n = 3 independent experiments). ( K ) Primary mouse bone marrow stromal cells from Lepr-Cre; Itga11 fl/fl or littermate control mice were adherently cultured in osteogenic differentiation medium. PBS (control), recombinant human Pro-Collagen 1α, or recombinant human Osteolectin was added then the cells were lysed 1 hr later and lysates were immunoblotted for phospho-FAK and FAK (this blot is representative of blots from two independent experiments). All numerical data reflect mean ±standard deviation. Statistical significance was determined with two-way ANOVAs with Tukey’s multiple comparisons tests ( A, B and D ), Dunnett’s multiple comparisons tests ( F ), or Sidak’s multiple comparisons tests ( J ). 10.7554/eLife.42274.010 Figure 4—source data 1. Data for .

Article Snippet: Antibody , goat polyclonal anti-mouse Osteolectin , R and D Systems , AF3729 , (1:1000).

Techniques: Recombinant, Quantitative RT-PCR, Cell Culture, Control, Membrane, Isolation, Centrifugation, Standard Deviation

( A ) Lepr-Cre; Itga11 fl/fl mice were grossly normal and indistinguishable from littermate controls. ( B – D ) Body length ( B ), body mass ( C ) and femur length ( D ) did not significantly differ between Lepr-Cre; Itga11 fl/fl mice and sex-matched littermate controls at 2, 6, or 12 months of age (n = 4–7 mice per genotype per sex per time point, from at least three independent experiments). ( E ) ELISA measurement of serum Osteolectin levels in Lepr-Cre; Itga11 fl/fl mice and littermate controls at 2, 6, and 12 months of age (n = 8 mice per genotype per time point from four independent experiments). ( F ) Representative microCT images of trabecular bone in the distal femur metaphysis of male Lepr-Cre; Itga11 fl/fl mice and littermate controls at 2, 6, and 12 months of age. ( G–L ) microCT analysis of trabecular bone volume/total volume ( G ), trabecular number ( H ), trabecular bone thickness ( I ), trabecular bone spacing ( J ), connectivity density ( K ), and bone mineral density ( L ) in the distal femur metaphysis of Lepr-Cre; Itga11 fl/fl mice and sex-matched littermate controls at 2, 6, and 12 months of age (n = 4–7 mice per genotype per sex per time point from at least three independent experiments). ( M ) Trabecular bone mineral apposition rate based on calcein double labelling in the distal femur metaphysis (n = 3 mice per genotype per sex per time point). ( N ) Bone resorption rate analysis by measuring the deoxypyridinoline/creatinine ratio in the urine of LepR-Cre; Itga11 fl/fl mice and littermate controls at 6 and 12 months of age (n = 4–5 mice per genotype per time point from three independent experiments). ( O ) Serum P1NP levels in LepR-Cre; Itga11 fl/fl mice and sex-matched littermate controls at 6 and 12 months of age (n = 4 mice per genotype per time point from three independent experiments). All numerical data reflect mean ±standard deviation. The statistical significance of differences was determined with two-way ANOVAs with Sidak’s multiple comparisons tests. 10.7554/eLife.42274.012 Figure 5—source data 1. Data for Data for .

Journal: eLife

Article Title: Integrin alpha11 is an Osteolectin receptor and is required for the maintenance of adult skeletal bone mass

doi: 10.7554/eLife.42274

Figure Lengend Snippet: ( A ) Lepr-Cre; Itga11 fl/fl mice were grossly normal and indistinguishable from littermate controls. ( B – D ) Body length ( B ), body mass ( C ) and femur length ( D ) did not significantly differ between Lepr-Cre; Itga11 fl/fl mice and sex-matched littermate controls at 2, 6, or 12 months of age (n = 4–7 mice per genotype per sex per time point, from at least three independent experiments). ( E ) ELISA measurement of serum Osteolectin levels in Lepr-Cre; Itga11 fl/fl mice and littermate controls at 2, 6, and 12 months of age (n = 8 mice per genotype per time point from four independent experiments). ( F ) Representative microCT images of trabecular bone in the distal femur metaphysis of male Lepr-Cre; Itga11 fl/fl mice and littermate controls at 2, 6, and 12 months of age. ( G–L ) microCT analysis of trabecular bone volume/total volume ( G ), trabecular number ( H ), trabecular bone thickness ( I ), trabecular bone spacing ( J ), connectivity density ( K ), and bone mineral density ( L ) in the distal femur metaphysis of Lepr-Cre; Itga11 fl/fl mice and sex-matched littermate controls at 2, 6, and 12 months of age (n = 4–7 mice per genotype per sex per time point from at least three independent experiments). ( M ) Trabecular bone mineral apposition rate based on calcein double labelling in the distal femur metaphysis (n = 3 mice per genotype per sex per time point). ( N ) Bone resorption rate analysis by measuring the deoxypyridinoline/creatinine ratio in the urine of LepR-Cre; Itga11 fl/fl mice and littermate controls at 6 and 12 months of age (n = 4–5 mice per genotype per time point from three independent experiments). ( O ) Serum P1NP levels in LepR-Cre; Itga11 fl/fl mice and sex-matched littermate controls at 6 and 12 months of age (n = 4 mice per genotype per time point from three independent experiments). All numerical data reflect mean ±standard deviation. The statistical significance of differences was determined with two-way ANOVAs with Sidak’s multiple comparisons tests. 10.7554/eLife.42274.012 Figure 5—source data 1. Data for Data for .

Article Snippet: Antibody , goat polyclonal anti-mouse Osteolectin , R and D Systems , AF3729 , (1:1000).

Techniques: Enzyme-linked Immunosorbent Assay, Standard Deviation

( A ) CFU-F frequency and ( B ) cells per CFU-F colony formed by bone marrow cells from Lepr-Cre; Itga11 fl/fl mice and littermate controls at 2 and 6 months of age (n = 6–8 mice per genotype per time point from at least three independent experiments). ( C–E ) Osteogenic ( C ); n = 7 mice per genotype, total, from seven independent experiments), adipogenic ( D ); n = 6 mice per genotype, total, from six independent experiments), and chondrogenic ( E ); n = 5 mice per genotype, total, from five independent experiments) differentiation of bone marrow stromal cells cultured from the femurs of Lepr-Cre; Itga11 fl/fl mice and sex-matched littermate controls at 2 months of age. ( F ) Recombinant mouse Osteolectin promoted osteogenic differentiation in culture by femur bone marrow stromal cells from control but not Lepr-Cre; Itga11 fl/fl mice (n = 3 mice per genotype, total, from three independent experiments). ( G ) qRT-PCR analysis of Dmp1 transcript levels in cells from panel ( F ). ( H–N ) Subcutaneous injection of recombinant mouse Osteolectin daily for 28 days (50 μg/kg body mass/day) significantly increased trabecular bone volume and number in female control mice but not female Lepr-Cre; Itga11 fl/fl mice. ( H ) Representative microCT images of trabecular bone in the distal femur metaphysis. ( I–N ) microCT analysis of trabecular bone volume/total volume ( I ), trabecular number ( J ), trabecular thickness ( K ), trabecular spacing ( L ), connectivity density ( M ), and bone mineral density ( N ) in the distal femur metaphysis (n = 5 mice per treatment, total, from three independent experiments). ( O ) The GSK3 inhibitor, AZD2858, rescued the osteogenic differentiation in culture of bone marrow stromal cells from the femurs of Lepr-Cre; Itga11 fl/fl mice (n = 6 independent experiments in which cells from one mouse of each genotype were cultured in each experiment). ( P ) Western blotting of cultured cell lysates showed that AZD2858 promoted GSK3 phosphorylation and increased β-catenin levels in bone marrow stromal cells from Lepr-Cre; Itga11 fl/fl mice and littermate controls. All numerical data reflect mean ±standard deviation. The statistical significance of differences was determined with Wilcoxon’s test followed by Holm-Sidak multiple comparisons adjustment ( A and B ), one-way ( F ), ( G, ) and O ) ANOVAs with Sidak’s multiple comparisons tests, by paired t-tests ( C–E ), or by one-way ANOVAs with Tukey’s multiple comparisons tests ( I–N ). 10.7554/eLife.42274.018 Figure 7—source data 1. Data for .

Journal: eLife

Article Title: Integrin alpha11 is an Osteolectin receptor and is required for the maintenance of adult skeletal bone mass

doi: 10.7554/eLife.42274

Figure Lengend Snippet: ( A ) CFU-F frequency and ( B ) cells per CFU-F colony formed by bone marrow cells from Lepr-Cre; Itga11 fl/fl mice and littermate controls at 2 and 6 months of age (n = 6–8 mice per genotype per time point from at least three independent experiments). ( C–E ) Osteogenic ( C ); n = 7 mice per genotype, total, from seven independent experiments), adipogenic ( D ); n = 6 mice per genotype, total, from six independent experiments), and chondrogenic ( E ); n = 5 mice per genotype, total, from five independent experiments) differentiation of bone marrow stromal cells cultured from the femurs of Lepr-Cre; Itga11 fl/fl mice and sex-matched littermate controls at 2 months of age. ( F ) Recombinant mouse Osteolectin promoted osteogenic differentiation in culture by femur bone marrow stromal cells from control but not Lepr-Cre; Itga11 fl/fl mice (n = 3 mice per genotype, total, from three independent experiments). ( G ) qRT-PCR analysis of Dmp1 transcript levels in cells from panel ( F ). ( H–N ) Subcutaneous injection of recombinant mouse Osteolectin daily for 28 days (50 μg/kg body mass/day) significantly increased trabecular bone volume and number in female control mice but not female Lepr-Cre; Itga11 fl/fl mice. ( H ) Representative microCT images of trabecular bone in the distal femur metaphysis. ( I–N ) microCT analysis of trabecular bone volume/total volume ( I ), trabecular number ( J ), trabecular thickness ( K ), trabecular spacing ( L ), connectivity density ( M ), and bone mineral density ( N ) in the distal femur metaphysis (n = 5 mice per treatment, total, from three independent experiments). ( O ) The GSK3 inhibitor, AZD2858, rescued the osteogenic differentiation in culture of bone marrow stromal cells from the femurs of Lepr-Cre; Itga11 fl/fl mice (n = 6 independent experiments in which cells from one mouse of each genotype were cultured in each experiment). ( P ) Western blotting of cultured cell lysates showed that AZD2858 promoted GSK3 phosphorylation and increased β-catenin levels in bone marrow stromal cells from Lepr-Cre; Itga11 fl/fl mice and littermate controls. All numerical data reflect mean ±standard deviation. The statistical significance of differences was determined with Wilcoxon’s test followed by Holm-Sidak multiple comparisons adjustment ( A and B ), one-way ( F ), ( G, ) and O ) ANOVAs with Sidak’s multiple comparisons tests, by paired t-tests ( C–E ), or by one-way ANOVAs with Tukey’s multiple comparisons tests ( I–N ). 10.7554/eLife.42274.018 Figure 7—source data 1. Data for .

Article Snippet: Antibody , goat polyclonal anti-mouse Osteolectin , R and D Systems , AF3729 , (1:1000).

Techniques: Cell Culture, Recombinant, Control, Quantitative RT-PCR, Injection, Western Blot, Phospho-proteomics, Standard Deviation

( A ) Bone marrow stromal cells from Lepr-Cre; Itga11 fl/fl mice and sex-matched littermates at 2 months of age were cultured in osteogenic differentiation medium supplemented with PBS (control) or 30 ng/ml Osteolectin and qRT-PCR analysis of Wnt target gene transcript levels was performed 24 hr later (n = 3 independent experiments). ( B ) Recombinant mouse Osteolectin subcutaneously injected daily for 28 days (50 μg/kg body mass/day) increased Wnt target gene transcript levels in LepR + bone marrow cells from control, but not Lepr-Cre; Itga11 fl/fl , mice (n = 4 mice per genotype per time point from two independent experiments). All numerical data reflect mean ±standard deviation. Statistical significance was assessed with two-way ANOVAs with Tukey’s multiple comparisons tests ( A ) or Dunnett’s multiple comparisons tests ( B ). 10.7554/eLife.42274.020 Figure 7—figure supplement 1—source data 1. Data for .

Journal: eLife

Article Title: Integrin alpha11 is an Osteolectin receptor and is required for the maintenance of adult skeletal bone mass

doi: 10.7554/eLife.42274

Figure Lengend Snippet: ( A ) Bone marrow stromal cells from Lepr-Cre; Itga11 fl/fl mice and sex-matched littermates at 2 months of age were cultured in osteogenic differentiation medium supplemented with PBS (control) or 30 ng/ml Osteolectin and qRT-PCR analysis of Wnt target gene transcript levels was performed 24 hr later (n = 3 independent experiments). ( B ) Recombinant mouse Osteolectin subcutaneously injected daily for 28 days (50 μg/kg body mass/day) increased Wnt target gene transcript levels in LepR + bone marrow cells from control, but not Lepr-Cre; Itga11 fl/fl , mice (n = 4 mice per genotype per time point from two independent experiments). All numerical data reflect mean ±standard deviation. Statistical significance was assessed with two-way ANOVAs with Tukey’s multiple comparisons tests ( A ) or Dunnett’s multiple comparisons tests ( B ). 10.7554/eLife.42274.020 Figure 7—figure supplement 1—source data 1. Data for .

Article Snippet: Antibody , goat polyclonal anti-mouse Osteolectin , R and D Systems , AF3729 , (1:1000).

Techniques: Cell Culture, Control, Quantitative RT-PCR, Recombinant, Injection, Standard Deviation

Journal: eLife

Article Title: Integrin alpha11 is an Osteolectin receptor and is required for the maintenance of adult skeletal bone mass

doi: 10.7554/eLife.42274

Figure Lengend Snippet:

Article Snippet: Antibody , goat polyclonal anti-mouse Osteolectin , R and D Systems , AF3729 , (1:1000).

Techniques: Recombinant, Diagnostic Assay, Cell Culture, Protease Inhibitor, Western Blot, Reverse Transcription, Enzyme-linked Immunosorbent Assay, Fractionation

$RNA-seq analysis of LepR + cells from non-irradiated bone marrow as well as hematopoietic cells and unfractionated cells from irradiated bone marrow (which are high in adipocytes). Itga11 is poorly expressed in adipocyte-rich bone marrow cells after irradiation (all data represent average FPKM values from multiple replicates).$

Journal: eLife

Article Title: Integrin alpha11 is an Osteolectin receptor and is required for the maintenance of adult skeletal bone mass

doi: 10.7554/eLife.42274

Figure Lengend Snippet: RNA-seq analysis of LepR + cells from non-irradiated bone marrow as well as hematopoietic cells and unfractionated cells from irradiated bone marrow (which are high in adipocytes). Itga11 is poorly expressed in adipocyte-rich bone marrow cells after irradiation (all data represent average FPKM values from multiple replicates).

Article Snippet: Antibody , goat polyclonal anti-mouse Osteolectin , R and D Systems , AF3729 , (1:1000).

Techniques: Irradiation

Figure 1. Clec11a deficient mice were grossly developmentally normal and had normal hematopoiesis. (A–C) Clec11a expression analysis by microarray, RNA-seq and qRT-PCR. Whole bone marrow cells, VE-Cadherin+ bone marrow endothelial cells, bone marrow stromal cells (Scf- GFP+CD45-Ter119-CD31- for microarray, PDGFRa+CD45-Ter119-CD31- for RNA-seq, and LepR+CD45-Ter119-CD31- for qPCR), Col2.3- GFP+CD45-Ter119-CD31- osteoblasts and hematopoietic cell populations were sorted from enzymatically dissociated femur bone marrow of two Figure 1 continued on next page

Journal: eLife

Article Title: Clec11a/osteolectin is an osteogenic growth factor that promotes the maintenance of the adult skeleton

doi: 10.7554/elife.18782

Figure Lengend Snippet: Figure 1. Clec11a deficient mice were grossly developmentally normal and had normal hematopoiesis. (A–C) Clec11a expression analysis by microarray, RNA-seq and qRT-PCR. Whole bone marrow cells, VE-Cadherin+ bone marrow endothelial cells, bone marrow stromal cells (Scf- GFP+CD45-Ter119-CD31- for microarray, PDGFRa+CD45-Ter119-CD31- for RNA-seq, and LepR+CD45-Ter119-CD31- for qPCR), Col2.3- GFP+CD45-Ter119-CD31- osteoblasts and hematopoietic cell populations were sorted from enzymatically dissociated femur bone marrow of two Figure 1 continued on next page

Article Snippet: Sections were blocked in PBS with 10% horse serum for 30 min and then stained overnight at 4 ̊C with goat IgG control (R and D Systems, 1:500), goat anti-Clec11a antibody (R and D systems, 1:500), rabbit anti-Aggrecan antibody (Chemicon, 1:500), rabbit anti-Per- ilipin antibody (Sigma, 1:2000) or goat anti-Osteopontin antibody (R and D, 1:500).

Techniques: Expressing, Microarray, RNA Sequencing, Quantitative RT-PCR

Figure 2. Clec11a is necessary for osteogenesis in limb bones and vertebrae. (A–C) MicroCT images of trabecular bone in the distal femur metaphysis of two month-old (A), 10 month-old (B) and 16 month-old (C) Clec11a-/- mice and sex-matched littermate controls. (D–I) MicroCT analysis of trabecular bone volume/total volume (D), trabecular number (E), trabecular thickness (F), trabecular spacing (G), connectivity density (H) and bone mineral density (I)) in the distal femur metaphysis of 2, 10 and 16 month-old Clec11a-/- mice and sex-matched littermate controls (n = 4–9 mice/genotype from at least Figure 2 continued on next page

Journal: eLife

Article Title: Clec11a/osteolectin is an osteogenic growth factor that promotes the maintenance of the adult skeleton

doi: 10.7554/elife.18782

Figure Lengend Snippet: Figure 2. Clec11a is necessary for osteogenesis in limb bones and vertebrae. (A–C) MicroCT images of trabecular bone in the distal femur metaphysis of two month-old (A), 10 month-old (B) and 16 month-old (C) Clec11a-/- mice and sex-matched littermate controls. (D–I) MicroCT analysis of trabecular bone volume/total volume (D), trabecular number (E), trabecular thickness (F), trabecular spacing (G), connectivity density (H) and bone mineral density (I)) in the distal femur metaphysis of 2, 10 and 16 month-old Clec11a-/- mice and sex-matched littermate controls (n = 4–9 mice/genotype from at least Figure 2 continued on next page

Techniques:

Figure 3. Clec11a is necessary for osteogenic differentiation. (A–D) Osteogenic differentiation in culture of bone marrow stromal cells from the femur bone marrow of Clec11-/- mice and sex-matched littermate controls. Alkaline phosphatase staining and alizarin red staining were performed after seven days (A and B) and 14 days (C and D) to quantify osteoblast differentiation and mineralization (n = 3 independent experiments). (E and F) Adipogenic differentiation in culture of bone marrow stromal cells from the femur bone marrow of Clec11-/- mice and sex-matched littermate controls. Oil red O staining was performed after four days (n = 3 independent experiments). (G and H) Chondrogenic differentiation in cell pellets of bone marrow stromal cells from the femur bone marrow of Clec11-/- mice and sex-matched littermate controls. Toluidine blue staining was performed on cryosectioned cell pellets after 21 days (n = 3 independent experiments). (I–K) Representative perilipin and osteopontin (OPN) staining in femur sections of two month-old Clec11-/- mice and sex-matched littermate controls (I and J) as well as the number of adipocytes per mm2 in sections through the bone marrow metaphysis (K) (n = 3 mice per genotype, total, from three independent experiments). (L–N) Representative Safranin O/fast green staining in femur sections of two month-old Clec11a-/- mice and sex-matched littermate controls (L and M) as well as the number of chondrocytes per mm2 in sections through the growth plate (N) (n = 3 mice per genotype from three independent experiments). The statistical significance of differences among genotypes was assessed using two-tailed Student’s t tests. All data represent mean ± SD (**p<0.01, ***p<0.001) from female mice. The source data are in Figure 3—source data 1. DOI: 10.7554/eLife.18782.008 The following source data and figure supplements are available for figure 3:

Journal: eLife

Article Title: Clec11a/osteolectin is an osteogenic growth factor that promotes the maintenance of the adult skeleton

doi: 10.7554/elife.18782

Figure Lengend Snippet: Figure 3. Clec11a is necessary for osteogenic differentiation. (A–D) Osteogenic differentiation in culture of bone marrow stromal cells from the femur bone marrow of Clec11-/- mice and sex-matched littermate controls. Alkaline phosphatase staining and alizarin red staining were performed after seven days (A and B) and 14 days (C and D) to quantify osteoblast differentiation and mineralization (n = 3 independent experiments). (E and F) Adipogenic differentiation in culture of bone marrow stromal cells from the femur bone marrow of Clec11-/- mice and sex-matched littermate controls. Oil red O staining was performed after four days (n = 3 independent experiments). (G and H) Chondrogenic differentiation in cell pellets of bone marrow stromal cells from the femur bone marrow of Clec11-/- mice and sex-matched littermate controls. Toluidine blue staining was performed on cryosectioned cell pellets after 21 days (n = 3 independent experiments). (I–K) Representative perilipin and osteopontin (OPN) staining in femur sections of two month-old Clec11-/- mice and sex-matched littermate controls (I and J) as well as the number of adipocytes per mm2 in sections through the bone marrow metaphysis (K) (n = 3 mice per genotype, total, from three independent experiments). (L–N) Representative Safranin O/fast green staining in femur sections of two month-old Clec11a-/- mice and sex-matched littermate controls (L and M) as well as the number of chondrocytes per mm2 in sections through the growth plate (N) (n = 3 mice per genotype from three independent experiments). The statistical significance of differences among genotypes was assessed using two-tailed Student’s t tests. All data represent mean ± SD (**p<0.01, ***p<0.001) from female mice. The source data are in Figure 3—source data 1. DOI: 10.7554/eLife.18782.008 The following source data and figure supplements are available for figure 3:

Techniques: Staining, Two Tailed Test

$Figure 4. Clec11a is necessary for bone regeneration and fracture healing. (A and B) Hematoxylin and eosin (A; dark blue = bone marrow cells; pink = bone) and Safranin O (B; red = cartilage) staining of the callus around the fracture site two weeks after bone fracture. F, fibrous tissue. BM, bone marrow. C, cartilage. (C and D) Representative microCT snapshot images of the callus (C) and cut-plane images around the fracture site (D) two weeks after bone fracture. (E–M) MicroCT analysis of trabecular bone volume/total volume (E), trabecular number (F), trabecular thickness (G), connectivity density (H), trabecular spacing (I), bone mineral density (J), callus volume (K), callus diameter (L) and polar moment of inertia (M) in the callus two weeks after bone fracture (n = 3 mice per genotype, total, from three independent experiments). The statistical significance of differences was assessed using two-tailed Student’s t tests. All data represent mean ± SD (*p<0.05, **p<0.01) from male mice that were two months old at the time of fracture. The source data are in Figure 4—source data 1. DOI: 10.7554/eLife.18782.012 The following source data is available for figure 4:$

Journal: eLife

Article Title: Clec11a/osteolectin is an osteogenic growth factor that promotes the maintenance of the adult skeleton

doi: 10.7554/elife.18782

Figure Lengend Snippet: Figure 4. Clec11a is necessary for bone regeneration and fracture healing. (A and B) Hematoxylin and eosin (A; dark blue = bone marrow cells; pink = bone) and Safranin O (B; red = cartilage) staining of the callus around the fracture site two weeks after bone fracture. F, fibrous tissue. BM, bone marrow. C, cartilage. (C and D) Representative microCT snapshot images of the callus (C) and cut-plane images around the fracture site (D) two weeks after bone fracture. (E–M) MicroCT analysis of trabecular bone volume/total volume (E), trabecular number (F), trabecular thickness (G), connectivity density (H), trabecular spacing (I), bone mineral density (J), callus volume (K), callus diameter (L) and polar moment of inertia (M) in the callus two weeks after bone fracture (n = 3 mice per genotype, total, from three independent experiments). The statistical significance of differences was assessed using two-tailed Student’s t tests. All data represent mean ± SD (*p<0.05, **p<0.01) from male mice that were two months old at the time of fracture. The source data are in Figure 4—source data 1. DOI: 10.7554/eLife.18782.012 The following source data is available for figure 4:

Techniques: Staining, Two Tailed Test

Figure 5. Recombinant Clec11a promotes osteogenesis in vitro and in vivo. (A and B) Osteogenic differentiation of stromal cells from femur bone marrow of wild-type mice. Vehicle or 10 ng/ml rClec11a were added to osteogenic culture conditions and alizarin red staining was assessed 14 days later to test whether Clec11a would promote osteogenesis (n = 3 independent experiments with duplicate cultures per treatment per experiment). (C) Representative microCT images of trabecular bone in the distal femur metaphysis of wild-type female mice treated with daily subcutaneous doses of Figure 5 continued on next page

Journal: eLife

Article Title: Clec11a/osteolectin is an osteogenic growth factor that promotes the maintenance of the adult skeleton

doi: 10.7554/elife.18782

Figure Lengend Snippet: Figure 5. Recombinant Clec11a promotes osteogenesis in vitro and in vivo. (A and B) Osteogenic differentiation of stromal cells from femur bone marrow of wild-type mice. Vehicle or 10 ng/ml rClec11a were added to osteogenic culture conditions and alizarin red staining was assessed 14 days later to test whether Clec11a would promote osteogenesis (n = 3 independent experiments with duplicate cultures per treatment per experiment). (C) Representative microCT images of trabecular bone in the distal femur metaphysis of wild-type female mice treated with daily subcutaneous doses of Figure 5 continued on next page

Techniques: Recombinant, In Vitro, In Vivo, Staining

Figure 6. Recombinant Clec11a prevents osteoporosis. (A) Representative microCT images of trabecular bone in the distal femur metaphysis. Two month-old sham operated (Mock) or ovariectomized (OVX) female mice received daily subcutaneous injections with vehicle, 40 mg/kg human PTH, or 50 mg/kg rClec11a for 28 days. (B–E) MicroCT analysis of trabecular bone parameters in the distal femur metaphysis of the mice from the experiment in panel A (n = 6–8 mice per treatment, total, from six independent experiments). (F) Bone resorption analysis based on the deoxypyridinoline/creatinine Figure 6 continued on next page

Journal: eLife

Article Title: Clec11a/osteolectin is an osteogenic growth factor that promotes the maintenance of the adult skeleton

doi: 10.7554/elife.18782

Figure Lengend Snippet: Figure 6. Recombinant Clec11a prevents osteoporosis. (A) Representative microCT images of trabecular bone in the distal femur metaphysis. Two month-old sham operated (Mock) or ovariectomized (OVX) female mice received daily subcutaneous injections with vehicle, 40 mg/kg human PTH, or 50 mg/kg rClec11a for 28 days. (B–E) MicroCT analysis of trabecular bone parameters in the distal femur metaphysis of the mice from the experiment in panel A (n = 6–8 mice per treatment, total, from six independent experiments). (F) Bone resorption analysis based on the deoxypyridinoline/creatinine Figure 6 continued on next page

Techniques: Recombinant

Figure 7. Recombinant Clec11a reverses osteoporosis and promotes osteogenesis by human bone marrow stromal cells. (A) Representative microCT images of trabecular bone in the distal femur metaphysis. Two month-old sham operated (Mock) or ovariectomized (OVX) female mice were left untreated for 28 days for osteoporosis to develop, and then received daily subcutaneous injections with vehicle, 40 mg/kg human PTH, or 50 mg/kg rClec11a for another 28 days. (B–G) MicroCT analysis of trabecular bone parameters in the distal femur metaphysis of the mice from the experiment in Figure 7 continued on next page

Journal: eLife

Article Title: Clec11a/osteolectin is an osteogenic growth factor that promotes the maintenance of the adult skeleton

doi: 10.7554/elife.18782

Figure Lengend Snippet: Figure 7. Recombinant Clec11a reverses osteoporosis and promotes osteogenesis by human bone marrow stromal cells. (A) Representative microCT images of trabecular bone in the distal femur metaphysis. Two month-old sham operated (Mock) or ovariectomized (OVX) female mice were left untreated for 28 days for osteoporosis to develop, and then received daily subcutaneous injections with vehicle, 40 mg/kg human PTH, or 50 mg/kg rClec11a for another 28 days. (B–G) MicroCT analysis of trabecular bone parameters in the distal femur metaphysis of the mice from the experiment in Figure 7 continued on next page

Techniques: Recombinant

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goat anti-clec11a antibody (R&D Systems)

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